dc.contributor.author | Tandogan, Berivan | |
dc.contributor.author | Kuruuzum-Uz, Ayse | |
dc.contributor.author | Sengezer, Cihangir | |
dc.contributor.author | Guvenalp, Zuhal | |
dc.contributor.author | Demirezer, L. Omur | |
dc.contributor.author | Ulusu, N. Nuray | |
dc.date.accessioned | 2019-12-16T10:29:22Z | |
dc.date.available | 2019-12-16T10:29:22Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 1388-0209 | |
dc.identifier.uri | https://doi.org/10.3109/13880209.2010.533187 | |
dc.identifier.uri | http://hdl.handle.net/11655/20106 | |
dc.description.abstract | Context: Glutathione reductase (GR, NADPH:oxidized glutathione oxidoreductase, E. C 1.6.4.2) is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG). GR is a crucial enzyme in the antioxidant system by maintaining reduced glutathione (GSH). Glucose 6-phosphate dehydrogenase (G6PD, glucose 6-phosphate (G6P):NADP(+) oxidoreductase, EC 1.1.1.49) is the key regulatory enzyme of the pentose phosphate pathway and maintains NADPH for reductive reactions. Objective: Rosmarinic acid (RA; alpha alpha-O-caffeoyl-3,4-dihydroxyphenyl lactic acid) is an ester of caffeic acid (CA) and 3,4-dihydroxyphenyllactic acid. It has a number of interesting biological activities. The inhibiting activities of the RA on GR and G6PD are investigated here for the first time. Materials and methods: GR and G6PD were purified from tissues, then the effects of RA are investigated. Results: This study reports that RA, which was isolated from Echium vulgare L. (Boraginaceae), inhibits purified GR and G6PD in a concentration-dependent manner. Kinetic characterizations and inhibition constants are investigated. Discussion and conclusion: Because of their importance in the antioxidative defense system, investigation of the inhibitors of these enzymes is important for drug development. | |
dc.language.iso | en | |
dc.publisher | Taylor & Francis Ltd | |
dc.relation.isversionof | 10.3109/13880209.2010.533187 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Plant Sciences | |
dc.subject | Medical Laboratory Technology | |
dc.subject | Pharmacology & Pharmacy | |
dc.title | In Vitro Effects Of Rosmarinic Acid On Glutathione Reductase And Glucose 6-Phosphate Dehydrogenase | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.relation.journal | Pharmaceutical Biology | |
dc.contributor.department | Biyokimya | |
dc.identifier.volume | 49 | |
dc.identifier.issue | 6 | |
dc.identifier.startpage | 587 | |
dc.identifier.endpage | 594 | |
dc.description.index | WoS | |
dc.description.index | Scopus | |