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dc.contributor.authorCelik, Eda
dc.contributor.authorOllis, Anne A.
dc.contributor.authorLasanajak, Yi
dc.contributor.authorFisher, Adam C.
dc.contributor.authorGur, Goksu
dc.contributor.authorSmith, David F.
dc.contributor.authorDeLisa, Matthew P.
dc.date.accessioned2019-12-16T09:57:10Z
dc.date.available2019-12-16T09:57:10Z
dc.date.issued2015
dc.identifier.issn1860-6768
dc.identifier.urihttps://doi.org/10.1002/biot.201400354
dc.identifier.urihttp://hdl.handle.net/11655/19845
dc.description.abstractGlycan microarrays have become a powerful platform to investigate the interactions of carbohydrates with a variety of biomolecules. However, the number and diversity of glycans available for use in such arrays represent a key bottleneck in glycan array fabrication. To address this challenge, we describe a novel glycan array platform based on surface patterning of engineered glycophages that display unique carbohydrate epitopes. Specifically, we show that glycophages are compatible with surface immobilization procedures and that phage-displayed oligosaccharides retain the ability to be recognized by different glycan-binding proteins (e.g. antibodies and lectins) after immobilization. A key advantage of glycophage arrays is that large quantities of glycophages can be produced biosynthetically from recombinant bacteria and isolated directly from bacterial supernatants without laborious purification steps. Taken together, the glycophage array technology described here should help to expand the diversity of glycan libraries and provide a complement to the existing toolkit for high-throughput analysis of glycan-protein interactions.
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.isversionof10.1002/biot.201400354
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiotechnology & Applied Microbiology
dc.titleGlycoarrays With Engineered Phages Displaying Structurally Diverse Oligosaccharides Enable High-Throughput Detection Of Glycan-Protein Interactions
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalBiotechnology Journal
dc.contributor.departmentBiyomühendislik
dc.identifier.volume10
dc.identifier.issue1
dc.identifier.startpage199
dc.identifier.endpage209
dc.description.indexWoS


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