dc.contributor.author | Erkekoglu, Pinar | |
dc.contributor.author | Zeybek, Naciye D. | |
dc.contributor.author | Giray, Belma K. | |
dc.contributor.author | Rachidi, Walid | |
dc.contributor.author | Kizilgun, Murat | |
dc.contributor.author | Hininger-Favier, Isabelle | |
dc.contributor.author | Favier, Alain | |
dc.contributor.author | Asan, Esin | |
dc.contributor.author | Hincal, Filiz | |
dc.date.accessioned | 2019-12-12T06:26:15Z | |
dc.date.available | 2019-12-12T06:26:15Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0959-9673 | |
dc.identifier.uri | https://doi.org/10.1111/iep.12059 | |
dc.identifier.uri | http://hdl.handle.net/11655/16366 | |
dc.description.abstract | This study was performed to determine the hepatotoxicity of di(2-ethylhexyl)phthalate (DEHP) in relation to selenium status. In 3-week-old Sprague-Dawley rats, selenium deficiency was induced by a 0.05 selenium mg/kg. A selenium supplementation group was given 1mg selenium/kg diet for 5weeks. Di(2-ethylhexyl)phthalate-treated groups received 1000mg/kg dose by gavage during the last 10days of the experiment. Histopathology, peroxisome proliferation, catalase (CAT) immunoreactivity and activity and apoptosis were assessed. Activities of antioxidant selenoenzymes [glutathione peroxidase 1 (GPx1), glutathione peroxidase 4 (GPx4), thioredoxin reductase (TrxR1)], superoxide dismutase (SOD), and glutathione S-transferase (GST); aminotransferase, total glutathione (tGSH), and lipid peroxidation (LP) levels were measured. Di(2-ethylhexyl)phthalate caused cellular disorganization while necrosis and inflammatory cell infiltration were observed in Se-deficient DEHP group (DEHP/SeD). Catalase activity and immunoreactivity were increased in all DEHP-treated groups. Glutathione peroxidase 1 and GPx4 activities decreased significantly in DEHP and DEHP/SeD groups, while GST activities decreased in all DEHP-exposed groups. Thioredoxin reductase activity increased in DEHP and DEHP/SeS, while total SOD activities increased in all DEHP-treated groups. Lipid peroxidation levels increased significantly in SeD (26%), DEHP (38%) and DEHP/SeD (71%) groups. Selenium supplementation partially ameliorated DEHP-induced hepatotoxicity; while in DEHP/SeD group, drastic changes in hepatic histopathology and oxidative stress parameters were observed. | |
dc.language.iso | en | |
dc.publisher | Wiley-Blackwell | |
dc.relation.isversionof | 10.1111/iep.12059 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Pathology | |
dc.title | The Effects of Di(2-Ethylhexyl)Phthalate on Rat Liver in Relation to Selenium Status | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.relation.journal | International Journal Of Experimental Pathology | |
dc.contributor.department | Histoloji ve Embriyoloji | |
dc.identifier.volume | 95 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 64 | |
dc.identifier.endpage | 77 | |
dc.description.index | WoS | |