Tıbbi Cihaz Uygulamalarına Bağlı Olarak Gelişen Biyofilm Bazlı Osteomiyelit Tedavisi Için Polimerik Ilaç Taşıyıcı Sistemlerin Formülasyonu Ve Değerlendirilmesi

Abstract

Medical device is a suitable substrate for bacteria in order to form biofilm which causes a high resistance to used antibiotics and slow or incomplete penetration of antibiotic to infected area, which makes the treatment of medical device-related (MDRO) osteomyelitis more difficult. Therefore, drug delivery system with direct injection to the infected site without surgery is required for the treatment of MDRO. Hence, breaking of biofilm and high antibiotic concentration which required for long term therapy could be provided. In this study vancomycin hydrochloride (VA) was encapsulated in low, medium and high molecular weight biocompatible-biodegradable polycaprolactone (PCL) polymer and is used to prepare VA encapsulated microspheres. After preparation of the microspheres are sterilized by gamma irradiation and before and after sterilization microspheres were characterized through particle size determination, encapsulation efficiency, surface morphology determinations, in vitro release studies, DSC thermograms and FT-IR spectrums. After these studies, thermosensitive chitosan and pluronic gels carrying serratiopeptidase (SP) enzyme, VA and VA loaded PCL microspheres were prepared and characterized through reological properties and in vitro VA and SP release properties. By the way, an in vitro biofilm model was developed and after the antibiofilm activity studies the most effective gel formulation was chosen for in vivo pilot studies. The results indicated that the biofilm, the most important part for the treatment of MDRO, is successfully broken by the chosen gel formulation.