İçi Boşluklu Gözenekli Silika Nanopartiküllerin İlaç Taşıyıcı Sistem Olarak Hazırlanması ve İncelenmesi

Abstract

In this thesis, designing a drug delivery system with high drug loading capacity due to it’s hollow interior, effective drug delivery by endosomal escape with proton-sponge effect and elongated blood circulation time to achieve a better Acute Myeloide Leukemia (AML) treatment is discussed. First of all, the characterization study was done to define mesoporous silica system. The average particle size was found as approximately 200nm by DLS measurements and this information checked by transmission electron microscopy (TEM) and the particles were approximately 150nm. Besides, it was observed that the hollow interior was achieved. It was observed that the outer wall of the particles were a crystal porous structure by X-Ray Diffraction, por diameter was approximately 16nm and the surface area was 232 m2/g by nitrogen adsorption-desorption isotherms (BET). The evaluated particles were charged negativly until chitosan- poly ethylene glycol (Chitosan-PEG) modification and after that the surface charge was positive which indicates chitosan-PEG was coated onto surface. The intact NPs have no toxic effect in vitro, yet the NPs with drug substance have a toxic effect almost as good as free doxubicin. The datas from in vivo studies showed that Chitosan-PEG coating was elongated blood circulation time of NPs. The designed system is promising for the treatment of AML