CD36 ve Lipid Peroksidasyonuna Diyetle Alınan Tekli Doymamış Yağ Asitleri, Doymuş Yağ Asitleri Ve Fruktozun Etkisi

Abstract

ABSTRACT YALÇİMİN ÖCAL, H., The Effect of Dietary Monounsaturated Fatty Acids, Saturated Fatty Acids and Fructose on CD36 and Lipid Peroxidation, Hacettepe University, Institute of Graduate School, Nutrition Sciences Program, Master Thesis, Ankara, 2019. Recent studies reported that increased consumption of refined sugar, intake of high amounts of saturated fatty acids and high fatty diets affect oxidant stress in the body. Disruption of the balance between pro-oxidants and antioxidants oxidative damage, lipid peroxidation therefore leads to chronic diseases. The aim of this study was to investigate the effects of high monounsaturated fatty acids (MUFA), saturated fatty acids (SFA) and fructose diets on lipid peroxidation and CD36 in plasma and tissues of mice. In the study in which samples were taken, C57BI/6 type eight weeks old male rats were divided into 4 groups as MUFA, SFA or fructose fed groups after 2 weeks of standardization. Mice were sacrificed after 15 weeks of dietary intervention and blood and tissues were drawn. According to the results of this study, lipid peroxidation marker TBARS (Thiobarbituric Acid Reactive Substances) levels were higher (p> 0.05) in plasma and tissues in the SFA group compared to the control group, but lower in MUFA group (p <0.05). In the fructose group, there was no difference in TBARS levels in plasma and tissues (p> 0.05). In addition, according to the results of the ELISA analysis, it was found that CD36 levels were higher in the heart and plasma in the SFA and fructose groups were compared to the control group but lower in MUFA-fed group (p <0.05). According to Western Blot analysis in heart tissue, whereas CD36 receptor levels were higher in fructose group, lower in MUFA group (p <0.05). These results support that balance by decreasing fructose and SFA consumption and by increasing MUFA consumption may have beneficial effects according to national and international guidelines for the prevention and treatment of chronic diseases. Keywords: Lipid peroxidation, CD36, mono unsaturated fatty acids, saturated fatty acids, fructose