Fare Ligaman Perforasyon SAK modelinde Squalene Adenozin Nanoparçaların Beyin Kan Akımı ve Nörolojik Skorlara Etkisi

Abstract

The aim of this study is to investigate the cerebral blood flow (CBF) changes in the first 24 hours following SAH, the effect of intravenous squalene-adenosine on CBF, functional outcome of mice and cerabral histologic findings in mice filament perforation SAH model. A total of 42 Swiss albino mice were used (6 in each of the following groups: control SAK, sham, squalene, low-dose adenosine, high-dose adenosine, low-dose squalene adenosine and high-dose squalene adenosine). SAH was induced in all groups other than Sham group. Laser speckle contrast imaging (LSCI) was used to measure bazal, immediate post SAH and 24th hour CBF leves. After 24 hours, locomotor activities were evaluated by open field test. The brains of the subjects who underwent functional evaluations were subjected to cresyl violet, TUNEL and Masson’s trichrome staining. In the early period after SAH, CBF was found to be decreased globally and severely in the bleeding groups. In the high dose squalene adenosine treatment group, CBF values were significantly higher than the control group. Similarly, the locomotor activity score obtained by open field test was higher in the group which received high dose squalene adenosine. Histological examinations revealed neuronal degeneration, apoptosis and intravascular fibrin plugs in all bleeding groups, which were less intense in the high-dose treatment group. In conclusion, it was observed that LSCI can accurately evaluate CBF changes after SAH. Intravenous application of high-dose squalene adenosine improves CBF after SAH, which in turn was reflected in functional evaluation.