Etanol Alımını Takiben Etil Glukuronid ve Etil Sülfat' ın Kan ve İdrar Konsantrasyonları Kinetiğinin Türk Toplumunda Gösterilmesi
Özet
Alcohol is the substance whose importance is increasing gradually in forensic medical science. Alcohol intake needs to be shown especially in criminal cases. Because of reducing to under the limit of detection, ethyl alcohol does not be determined in a short time in both blood and urine. It has been recently important to analyze ethyl glucuronide (EtG) and ethyl sulfate (EtS), minor metabolites of ethanol.
In our study, it was aimed to show kinetics of EtG and EtS in blood and urine after taking 0.5 g/kg ethanol. Therefore, it was the first time that these metabolites’ peak concentrations and time of vanishing were determined in Turkish people by an experimental study.
Seventeen (10 male, 7 female) healthy volunteers participated in the study. After taking 0.5 g/kg ethanol, blood and urine samples were collected during 48 hours. The samples were analyzed in LC-MS/MS.
Serum peak concentrations of EtG and EtS were found as 0.13-0.389 mg/L and 0.211 – 0.5 mg/L respectively. Levels of urine EtG peak concentrations was 6.89-30.42 mg/L and EtS was 10.5-58.17 mg/L. There was no EtG and EtS in all samples 24 hours later in blood and 48 hours later in urine. Additionally, correlation was found between serum EtG concentrations and serum EtS concentrations.
By this study conducted on Turkish population, determination times of EtG and EtS, and how long they can be used as a marker of alchohol intake after 0.5 g/kg ethanol intake were showed and results of the study were generally found parallel to the literature.
