Serbest Kalvaryal Periost ve Biyoaktif Cam Kullanılarak Neo-Osseöz Flep Prefabrikasyonu

Abstract

Reconstruction of the contour and functional deficiencies due to the absence of the bone tissue is among the most popular subjects Plastic Surgery deals with. In reconstructive surgery, autogeneous bone graft is considered as the best material to replace the damaged bone tissues after trauma or surgery. However, factors such as donor site morbidity, prolongation of operating time, difficulty in sculpting and the limited amount of donor tissue especially in pediatric population restrict the usage of autogenous bone grafts. Thus, biomaterials have become an integral part of the reconstructive surgery of the bone. Shorter operating time, ease of use and high resistance make biomaterials even more important. The primary aim of our study is to form a new bone (neo-osseous) tissue in the body, and to give extra volume and resistance to our newly formed bone tissue by using the biomaterial. In this study, 32 Wistar Albino Rats, supplied by the Hacettepe University Experimental Animals Unit, and which weigh 250-300 gr, were used. The biomaterial of choice to be used for the prefabrication of the flap was BonAlive® (BonAliveBiomaterials, Turku, Finland) bioactive glass, composed 53 % of silica dioxide, 23 % of sodium oxide, 20 % of calcium oxide and 4 % of diphosphopentaoxide. The subjects were allocated into 4 groups made of 8 subjects: Group 1 (FP) was the periosteum on the flap group, Group 2 (FPB) was the periosteum and bioactive glass on the flap group, Group 3 (FB) was the bioactive glass on the flap group, and Group 4 (F) was the flap-only group. Using 3 dimensional radiomorphometric analyses, the volumes of the areas with bone density were compared to the total flap volume in the subjects. The difference between the FPB and FB groups was statistically significant. Histologic examination revealed that lamellar bone formation was highest in the FP group, with a percent of 62,5 %, significantly higher than other groups. Also, according to the histological scoring, FP, FPB and FB groups all had a statistically important higher score compared to the control group.