Açıklanamayan İnfertilite Nedeniyle İntrauterin İnseminasyon Uygulanan Hastalarda Ejakülattaki Preapoptotik Sperm Oranının İntrauterin İnseminasyon Başarısındaki Etkisinin Araştırılması

Abstract

We hypothesis that the apoptotic sperm in ejaculate may be responsible for failure of intrauterine insemination in infertile couples and also may play a role on the etiology of unexplained infertility.This study includes the patients who consulted Hacettepe University Faculty of Medicine, Department of Obstetrics and Gynecology, Reproductive Endocrinology, Infertility and Andrology Unit between March 2012 - December 2012 and were undergoing ovulation induction and intrauterine insemination with diagnosis of unexplained infertility.In this study, the ejaculate from patients is stained with annexin V kit (Annexin V-FITC Apoptozis Detection Kit-Santa Cruz Biotechnology) after prepared by the density gradient centrifugation (DGS) method to determine the sperm proapoptotic rate and its relation to pregnancy outcome. The results of the patients who achieved pregnancy are compared to the results of the patients who couldn't achieve pregnancy. This study included 94 patients for whom ovulation induction was performed using rFSH due to unexplained infertility. The pregnancy rate of those 94 patients was 13.8% (n=13). The samples taken from subfertile men ejaculate were stained with annexin V after washing and were examined under immunofluorescence microscope. Annexin V-positive sperm rate was found as approximately 20.03% ± 13.73 (1-70). Annexin V-positive sperm rate has been found lesser among the patients who achieved pregnancy (15.69%) when compared to the patients who didn't achieve pregnancy (20.72%). However, the difference was not statically significant (p=0.17).