Burkholderia Türlerinin Moleküler Yöntemlerle Tiplendirilmesi, Türler Arasında Biyofilm Oluşumu ve Antibiyotik Direnç Farklılığının Belirlenmesi.

Abstract

The aim of this study was to identify Burkholderia spp. strains isolated from CF and non-CF patients with routine microbiological methods and multilocus sequence analysis (MLSA), to determine the antibiotic susceptibility and synergies, and to evaluate biofilm formation of these isolates. A total of 38 isolates from 26 patients identified as B. cenocepacia (n=16), B. contaminans (n=11), B. gladioli (n=4), B. dolosa (n=4), B. multivorans (n=2) and B. seminalis (n=1). Correct species-level identification of MALDI-TOF MS was 66%. Biofilm formation in these species was determined as 53% (n=20) and there was no statistical difference in biofilm production among Burkholderia species. Overall rates of susceptibility to ceftazidime, meropenem, TMP-SXT, and levofloxacin of the isolates was 66%, 34%, 50%and 60%, respectively. In antibiotic resistance against Burkholderia spp., isolates obtained from CF patients were more susceptible to ceftazidime, but no significant difference was found for other antibiotics. Synergy has shown between meropenem and TMP-SXT at two isolates. Antagonism has detected at 15 isolates, 12 of them were between meropenem and ceftazidime, three of them were between ceftazidime and TMP-SXT. This study identifies the distinctness of sequence types between Burkholderia spp. isolated from CF and non-CF patients provide a better understanding of the importance of biofilm formation for the infections with these bacteria and emphasize the management of therapy should be driven by the antibiotic test results.