Manyetik Özelliğe Sahip Monodispers-Gözenekli Silika Mikroküreler Üzerine Biyomolekül Baskılama Yoluyla Sorbent Sentezi ve Sorbentin Seçici Biyomolekül İzolasyonunda Kullanımı
Özet
In the scope of the thesis, monodisperse-porous silica microspheres used as support material for
selective sorbent synthesis for biomolecule isolation by molecular imprinting were synthesized
by multi-step hydrolysis / condensation method. For the magnetic form, iron oxide nanoparticles
were immobilized in silica microspheres. Subsequently, folic acid (FA) was imprinted
molecularly on the microspheres in both forms. After molecular imprinting, the sorbent was
washed away and the imprinted target molecule was removed from the structure and the
selective adsorption behavior of the obtained sorbent to target molecule (FA) was studied in a
batch and continuous system. Equilibrium adsorption capacity values (Q) were found and
imprinting factors (IF) were determined by scanning sorbent concentration and target molecule
concentration with molecularly imprinted microspheres (MMIP and MIP) and unimprinted
microspheres (MNIP and NIP) in magnetic and non-magnetic form in the batch system. At
optimal sorbent concentration, the Q values for MIP and NIP were 30.88 mg FA/g microspheres
and 7.57mg FA/g microspheres, respectively, and the IF value was 4.08.For MMIP and MNIP, the Q values were determined as 48.19mg FA/g microspheres and
17.57mg FA/g microspheres, so the IF value was 2.74.
The selectivity test showed that folic acid had a higher IF value in the batch adsorption system
than molecules of similar structure. It has been shown that MIP and MMIP sorbents can be used
in a reusable form in a batch system.
The size distribution and surface morphologies of the microspheres were determined by
scanning electron microscopy (SEM) images and the presence of the polymeric structure on the
microspheres after molecular imprinting was determined by images obtained from the
transmission electron microscope (TEM). Pore size and specific surface areas were determined
by nitrogen adsorption / desorption method (BET) and the magnetic behaviors of microspheres
in magnetic form were determined by vibrating sample magnetometer (VSM).
When the adsorption behavior of the continuous system was examined, it was observed that the
microcolumns containing MIP and NIP reached saturation in 6 and 3 minutes, respectively.
Reusability testing for the continuous system revealed that the microcolumn can be reused for
4 cycles. These results demonstrate that both the batch and the continuous system are suitable
for folic acid isolation and FA assay is successful with molecular imprinting.
