Antimikrobiyal Kompozit Polimerik Malzemelerin Tasarlanması

Abstract

In recent years, due to the development of antibiotic resistance by pathogenic microorganisms, there has been a trend towards the use of different antimicrobial agents and disinfectants. Among antimicrobial polymers, cryogels can be used in different fields of study thanks to their supermacroporous structure and their ability to be prepared with various monomers. In addition, boronic acid draws attention with its antimicrobial properties in the studies reported in the literature and is shown as an effective antimicrobial agent on pathogenic microorganisms. Nanotechnology is a branch of science that plays an important role in the development of antimicrobial polymers. Nanoparticles have also been widely used as antibacterial agents in the last decade. Their use for the inhibition of antibiotic-resistant bacteria is one of the most remarkable areas of use. In this thesis, poly(2-hydroxyethyl-methacrylate) (p(HEMA)), zinc oxide nanoparticle embedded poly(2-hydroxyethyl-methacrylate) (ZnO@p(HEMA)), The synthesis of vinyl phenyl boronic acid-functionalized hydroxyethyl methacrylate (p(HEMA-VPBA)) and zinc oxide (ZnO) nanoparticle embedded vinyl phenyl boronic acid (VBPA)-functionalized hydroxyethyl methacrylate (HEMA) based microcryogels ZnO@p(HEMA-VBPA) was carried out. The antimicrobial activities of the synthesized polymers were investigated using Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC 25923 strains as model microorganisms for Gram positive and Gram negative bacteria, respectively. Two methods were used to determine the antimicrobial activity. In the spectrophotometric method, the antimicrobial activities of the polymers included in the study were determined by measurements taken after 24, 48 and 72 hours of incubation. In this analysis, antimicrobial activity was calculated as relative bacterial inhibition. As a result, the lowest bacterial concentrations were observed for S. aureus ATCC 29213 and E. coli ATCC 25923 strains in cultures supplemented with ZnO@p(HEMA-VPBA). Relative bacterial inhibition was 92.7% for S. aureus ATCC 29213 at 48 and 72 hours. In the agar colony counting plate method, bacteria were inoculated from liquid media to solid media after serial dilutions from cultures formed after 24, 48 and 72 hours of incubation. After counting the bacterial colonies, cfu/mL values were calculated. It was observed that ZnO@p(HEMA-VPBA) microcryogel showed more antimicrobial effect on S. aureus ATCC 29213 strain, a Gram positive bacterium, than other synthesized microcryogels. Based on the cfu/mL values obtained, the bacterial retention capacity (Q) of microcryogels was calculated. According to the results of the experimental studies, the microcryogel with the highest bacterial retention capacity on the bacterial strains included in the study was ZnO@p(HEMA-VPBA). The bacterial retention capacity of ZnO@p(HEMA-VPBA) microcryogel was found to be 731000x105 cfu/g in the Gram positive bacteria S. aureus ATCC 29213 strain and 711300x105 cfu/g in the Gram negative bacteria E. coli ATCC 25923 strain. According to these values, it was observed that it was more effective on S.aureus ATCC 29213 strain compared to E.coli ATCC 25923 strain.