Farklı Toz-Sıvı Oranlarında Karıştırılmış Kalsiyum Hidroksit Patından İyon Salınımının İn Vitro Değerlendirilmesi

Abstract

DERINLER N., In Vitro Study of Ion Release from Calcium Hydroxide Paste Mixed at Different Powder to Liquid Ratios, Hacettepe University Faculty of Dentistry, Department of Endodontics, Specialization Thesis in Endodontics, Ankara, 2023 The purpose of our study was to evaluate the Ca+2 and OH- ion release from the apical foramen of calcium hydroxide paste mixed in different liquid carriers and at different ratios by weight. In this study, 105 single-canal human lower premolar teeth were used. The teeth were separated from their crowns and covered with 2 coats of nail polish, except for the apical foramen. The root canals were shaped with ProTaper Gold (Dentsply, Germany) rotary nickel titanium files up to F3 files. After the chemomechanical preparations, the teeth were randomly divided into 5 main groups (n=21). Each group was divided into 3 subgroups according to the determined concentrations (n=7). In our study, 30%, 40% and 50% calcium hydroxide powder by weight was mixed with saline, distilled water, chlorhexidine (CHX), sodium hypochlorite (NaOCl) and propylene glycol. The prepared calcium hydroxide paste was placed on the teeth with a lentulo with an apical diameter of #30. The cavities coronal to the prepared teeth were sealed with IRM (Dentsply, Germany) cement. The remaining coronal tooth area and cement were sealed with 2 coats of nail polish. The tooth roots prepared in our thesis study were placed in separate penicillin butyl bottles containing 8 ml of distilled water and specimens were prepared. They were kept at 35 ºC and 100% relative air humidity during the test period. After 1, 7, 14, 30 and 60 days, the distilled water in which the samples were placed was evaluated for pH and calcium ion release. The data obtained from the experiments were evaluated by repeated measures analysis of variance. Differential groups were determined by Bonferroni test. The pH and Ca+2 ion correlation between the groups was evaluated with Pearson's correlation coefficient. According to the results of our study, according to the pH measurement findings depending on the days and the carrier used, no significant difference was found on days 1, 7, 14, 30 and 60 in the groups with 30% concentration by weight (p>0.05). While no significant difference was found on days 7, 14, 30 and 60 in the groups with 40% concentration (p>0.05), the highest pH measurement findings were found on day 1. In the groups with 50% concentration, no significant difference was observed on days 1, 7, 14 and 30 (p>0.05), while the highest pH measurement finding on day 60 was observed in the groups in which propylene glycol was used as a carrier (p<0.05). In the pH measurement findings depending on the days and concentration, it was observed that the groups with higher concentrations increased the pH of the medium more as the number of days increased (p<0.05). According to the findings of Ca+2 ion concentration depending on the days and the carrier used, no significant difference was observed on days 7, 30 and 60 at 30% concentration (p>0.05), while the carrier group with the highest Ca+2 ion release on days 1 and 14 was propylene glycol (p<0.05). No significant difference was observed on day 30 at 40% concentration (p>0.05). At 50% concentration, no significant difference was observed on days 7, 14 and 30 (p>0.05), while the carrier group with the highest Ca+2 ion release on days 1 and 60 was propylene glycol (p<0.05). In the Ca+2 ion concentration findings depending on days and concentration, it was observed that the groups with higher concentration increased Ca+2 ion concentration more as the number of days increased (p<0.05). According to the correlation findings, it was observed that the correlation between pH and Ca+2 ion concentrations increased as the number of days increased. The day with the highest collinearity was the 30th day. In the light of the results of this thesis study, it was observed that the prepared calcium hydroxide paste caused more Ca+2 ion release in the medium as the number of days and concentration decreased, regardless of the carrier.