Akut Deri Yaraları İçin Bitki Türevli Çok Fonksiyonlu Kriyojellerin Geliştirilmesi

Abstract

Cryogels, today, are polymer materials that can find applications in various fields. Cryogels supported structurally by natural materials can serve as a supportive treatment modality in many areas such as wound healing, tissue scaffolding, and drug delivery. The aim of this doctoral study is to prepare a plant-derived cryogel utilizing the therapeutic effects of Melaleuca alternifolia oil metabolites and to use this plant-derived cryogel for rapid and effective intervention during incidents. For this purpose, biocompatible cryogels containing gelatin and alginate were prepared, and the aim was to enhance their antibacterial properties by incorporating tea tree oil into these cryogels. The synthesized cryogels were characterized using FTIR, swelling tests, SEM, and TGA. The incorporation of tea tree oil into the cryogel matrix was determined using GC-MS/HS. Additionally, the cumulative release of tea tree oil from the cryogels and the degradation of the cryogels were evaluated. The biocompatibility of the obtained cryogels was determined in vitro using HaCaT (Human Keratinocyte Cell Line) cells through an indirect MTT assay. At the end of the study, it was observed that tea tree oil was successfully loaded into alginate and gelatin cryogels, and its antibacterial activity was enhanced. The highest antibacterial activity was observed in the TTO100-ACry cryogel with a rate of 90,00 %. All cryogels were observed to be biocompatible, and among the gelatin groups, the TTO0-GCry group exhibited the highest cell viability with percentages of 146,2 % at 24 h and 151,4 % at 48 h. Among the alginate groups, the TTO100-ACry cryogel showed the highest cell viability with percentages of 100,0 % at 24 h and 106,0 % at 48 h. According to the scratch test results, the highest wound healing at 24 h was observed in the TTO0-GCry cryogel with 45,71 %. The highest wound healing was observed in the TTO100-ACry cryogel with 51,90 % at 48 h. According to the clotting analysis, the most suitable candidate for use as a rapid hemostatic agent in early intervention was TTO100-ACry with a percentage of 94,10% for the first 15 min. The cryogel supported by natural resources can be considered as a fast and effective hemostatic agent for wound healing, making it a pioneering approach for related studies. The methodology applied in this thesis, including the materials used, carries original value for the literature.