Bakteri Ayrılması için Moleküler Baskılama Temelli Kriyojel Membranlar

Abstract

Pseudomonas, an aerobic and gram-negative bacillus without fermenting properties, is widely found in soil, water and various moist environments. The type most commonly associated with disease in humans is Pseudomonas aeruginosa, with its opportunistic pathogen feature that can cause infection in almost any organ or tissue, especially in patients with another disease or immunocompromised. Unlike other aquatic pathogens, P. aeuginosa can even adapt to various temperature ranges and poor nutrient availability in the environment. Thanks to its polysaccharide capsule, it attaches to surfaces and rapidly increases its number in aquatic systems and forms a biofilm. Especially in large buildings such as hospitals, it can settle in water systems and cause contamination within the hospital. It is commonly found in environments where human activities are common. In addition, P. aeruginosa is found in the treated water, even after the purification processes for the production of drinking water, it is classified among the indicator bacteria. Due to all these features, the separation of P. aeruginosa from water sources is very important for the protection of public health. The design and development of materials with artificial molecular recognition properties is an active field with applications in fields as diverse as medicine, environmental monitoring, food safety and national security. Cryogels are materials consisting of a cross-linked hydrophilic polymer network with controlled macroporosity between 1-100 μm. As a result of polymerization to obtain cryogel, large pores and interconnected cryogel channels are formed. The high flow rate and fast mass transfer enable direct separation of cells without complex pretreatment processes, even from complex biological samples. Cryogels are the most suitable structures for the suppression and therefore removal of bacterial cells due to their supermacropores. In this study, solutions containing P. aeruginosa at different concentrations were removed by passing through cryogels. The structure and surface morphology of the cryogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and swelling experiments.