Rekombinant İnsan Hyaluronidazının (rHuPH20) Post Travmatik Ödeme Etkisinin Deksametazonla Karşılaştırmalı Olarak İncelenmesi

Abstract

Edema is a complication which may cause non-healing of epithelium lines, lessen the function and failing at the surgical operation. Today there are so many medications and ways to prevent edema due to surgical operations. But non of these ways and medications supresses the surgical edema at the desired level. At our study we aimed to reduce the operative edema which is named exudates of intercellular substance, by hyaluronidase enzyme which fasten the pass of the fluids from intercellular substance to systemic circulation. That activity of hyaluronidase enzyme is compared with deksamethasone’s which has the highest antiinflamatuar potency between glucocorticoids. As hyaluronidase enzyme, recombinant human hyaluronidase (rHuPH20) is used because of avoiding allergic reactions due to animal derived hyaluronidase preparats. 40 Sprague-Dawley albino rats divided randomly 5 equal groups. At 1. group just traumatic edema was made on 8 rats. At 2. group traumatic edema was made and 0.4 ml SF injected to the right hind paws of the rats. At 3. group 0.4 ml hyaluronidase injected to the right hind paws of the rats. At 4. group 0.4 ml deksamethasone injected to the right hind paws of the rats in the same way. At 5. group 0.4 ml deksamethasone-hyaluronidase combine injected to the right hind paws of the rats. The dosage of the medicines calculated according to weights of the rats. Because all fluids which are injected to the paws of the rats must be at the same volume, all medicines are completed to 0.4 ml by SF. All the rats’ right hind paws volume evaluated by plethysmometer before starting the traumatic edema formation and after it at the 3., 6., 12., 24., 48., 72., hours. As a result of statistical analysis deksamethasone didn’t significantly diminished the edema according to group 1, but significantly diminished the edema according to SF applicated group 2. Besides this, hyaluronidase significantly reduced the edema according to group 1 and 2. When hyaluronidase compared with dexamethasone, it had been shown hyaluronidase significantly reduces edema according to dexamethasone. Hyaluronidase-dexamethasone’s combine application provides less edema with regard to alone hyaluronidase application, but this difference isn’t significant. As a result of this study hyaluronidase enzyme may provide more better edema control than deksamethasone which is widely using effective agent for managing edema.