Kök Hücrelerde Olası DNA Hasarı ve Onarımının İn Vitro Değerlendirilmesi

Abstract

Stem cells provide an opportunity to analyse the effects of xenobiotic on cell viability, differentiation and cell functions. For this reason, it is suitable alternative for in vitro and in vivo methods. Evaluation of the possible cytotoxic and genotoxic effects on bone marrow CD34+ hematopoietic progenitor stem cells is important for differentation property of these cells into blood cells, and also for the diagnostic and the treatment processes of bone marrow diseases. In this thesis, boron nitride nanotubes which are commonly used but there is not enough information about its biocompatibility and curcumin which is being used frequently in treatment processes for antioxidant, antimicrobial, antiviral properties for a long time are selected to evaluate cytotoxic and genotoxic effects. The possible cytotoxic and genotoxic effects of boron nitride nanotubes and curcumin on bone marrow CD34+ hematopoietic progenitor stem cells were evaluated by MTT assay and COMET assay, respectively. Boron nitride nanotubes showed no significant cytotoxic effects on HeLa (human cervix adenocarcinoma) cells at the concentrations of 10-600 µg/ml and increased cell viability at 50-100 µg/ml and 400-600 µg/ml. Curcumin decreased cell viability proportionally with concentration. When they were used together, 50-100 µg/ml concentrations were suitable for cell viability. When the genotoxic effects of boron nitride nanotubes and curcumin were investigated, it is found that boron nitride nanotubes showed no correlation for concentration and DNA damage. Curcumin decreased DNA damage at high concentrations after 30 minutes of incubation and increased the damage after 24 hours of incubation. When they both took place in action, the DNA damage was lower than the single dosage groups of boron nitride nanotubes and curcumin.