Serviks Kanser Hücrelerinde Diklofenak Sodyumun Sisplatin Sitotoksisitesi Üzerine Etkilerinin İncelenmesi

Abstract

Avdan, F. Investigation of the Effects of Diclofenac Sodium on Cisplatin Cytotoxicity in Cervical Cancer Cells. Hacettepe University Graduate School of Health Sciences Master Thesis in Pharmaceutical Toxicology, Ankara, 2022. Cervical cancer is the second most common cancer in women and the third leading cause of cancer death after breast and lung cancer. The best possible treatment for cervical cancer is a combination of radiation and cisplatin-based chemotherapy. NSAIDs (nonsteroidal anti-inflammatory drugs) are best known for inhibiting cyclooxygenase (COX) enzymes that catalyze prostaglandin synthesis. Some epidemiological studies show that NSAIDs can reduce the incidence of cancer. NSAIDs have been shown to inhibit malignant transformation in various cancer cell lines, act as tumor suppressors, increase apoptosis, and enhance the cytotoxic activity of certain anticancer drugs. It is thought that diclofenac sodium, an NSAID drug, may have an effect on cisplatin cytotoxicity in human cervical cancer (HeLa) cells. Studies on the combined effects of cisplatin and diclofenac sodium in chemotherapy are insufficient. The aim of this study was to evaluate the cytotoxicity of cisplatin with diclofenac sodium combinations using MTT method and to clarify the anticancer effect. According to the study results, the IC50 values of diclofenac sodium for 24- and 48- hours incubation were found to be 679 μM and 238 μM in HeLa cell, respectively. The IC50 values of cisplatin for 24 and 48 hours incubation were found to be 24 μM and 10 μM, respectively. Diclofenac sodium significantly reduced the IC50 value of cisplatin in dose-dependent manner at the concentration ranges of 62,5 μM-1000 μM for 24-hour incubation and 31,3 μM-1000 μM for 48-hour incubation. In conclusion, our findings suggest that diclofenac sodium may increase the cytotoxic efficacy of cisplatin in cervical cancer treatment and may play an important role in cancer cell development and progression; however, further studies are needed to confirm the combined effects in anticancer drug treatment. Key Words: Diclofenac sodium, cisplatin, cytotoxicity, cervical cancer