Cytochrome C Purıfıcatıon with Surface Imprinted Bacterial Cellulose Nanofibers

Abstract

In the present study, cytochrome c imprinted bacterial cellulose (Cyt c-MIP)nanofibers were prepared for the purification and recognition of cytochrome c viasurface imprinting approach. N-methacryloyl-L-histidine methyl ester (MAH) wassynthesized to create specific binding sites. After the complexation between MAHand chelating metal ion copper(II), the preorganized complex was prepared withMAH-Cu(II) monomer and Cyt c template molecules via metal ion coordinationinteractions. Cyt c-MIP nanofibers were prepared in the presence of differentamounts of %w total monomer, monomer template ratio and polymerization time.Cyt c-MIP nanofibers were characterized by ATR-FTIR, SEM and contact anglemeasurements. In order to desorp the adsorbed proteins from the polymernetwork, 1 M NaCl solution was used. Adsorption studies were performed withrespect to pH, temperature and ionic strength and the adsorption capacity wasevaluated spectrophotometrically.