Polimerik Membranlardan Kontrollü İnsülin Salımının Kütle Spektrometrik Tayini

Abstract

The number of diabetes mellitus patients is increasing day by day in the world. This disease is caused by the failure or destruction of β cells responsible for insulin release. The most important task of the insulin hormone is to regulate carbohydrate metabolism. In addition, insulin hormone is needed for fat and protein metabolism. For this reason, diabetics have to take insulin to manage their illness. Current treatments include both psychological and physical risks. New treatments are needed to minimize these risks and improve the quality of life of patients. Chromatographic methods are frequently preferred in insulin hormone analysis. Within the scope of the thesis, the amount of insulin release from the insulin loaded polymeric membrane, depending on time, was measured using mass spectrometry following chromatographic techniques. Suitable solutions were determined that these membranes can release insulin under conditions compatible with the human body. It was aimed to contribute to the commercial production of polymeric membranes that can release insulin effectively by simply adhering to the human body. For this purpose, three different insulin loaded polymeric membranes were synthesized. The characterization studies of membranes containing hydroxypropyl methacrylate and hydroxypropyl methacrylate-chitosan have been carried out, and the surface morphology, particle size, temperature degradation behavior and polymerization properties of the membranes have been researched. MALDI-MS and ORBITRAP-MS analyzes, insulin release from membranes in three different solvents was observed qualitatively and quantitatively. As a result of this thesis, the presence of insülin in the membranes has been proved via the characterization studies. While the membrane containing hydroxypropyl methacrylate-chitosan-insulin was observed to release rapid and high amounts of insulin, it was found that the membrane containing hydroxypropyl methacrylate-insulin showed a slow and more stable insulin release behavior. Accordingly, the membrane containing hydroxypropyl methacrylate-insulin is considered to be more suitable in terms of usability.