Preparatıon of Poly(Glycıdyl Methacrylate) Baced Cryogels for Immunoaffınıty Chromatography
Özet
Immunoaffinity chromatography (IAC) is a special type of bioaffinity chromatography that uses antibodies or antibody-related molecules as the stationary phase. This technique is used by many disciplines, particularly prefering in biochemistry on one-step separation and purification of functional protein. Also, immunoaffinity chromatography is used in antibody enrichment and separation of cells. IAC columns typically have strong and selective binding between antibodies and their target antigens. Antigen or antibody immobilized to the solid support and target antibody or cell is purified. Specific bioligands can be immobilized directly on polymeric material with simple acid base catalyst. In our study, we prepared poly(glycidyl methacrylate) based cryogels and immobilized anti-insulin antibodies on porous surface of cryogels. We characterized cryogels with swelling test, Fourier transform infrared (FTIR) and Raman spectroscopy, and scanning electron microscopy. Also, effects of pH, i
