Nükleik Asit Bazları İçeren Kriyojellerin Sentezi, Karakterizasyonu ve Biyokromatografide Adsorbent Olarak Kullanımı

Abstract

In this study, development of adenine and guanine incorporated cryogels costly effective high capacity purification of large biomolecule RNA having many important roles as encoding, decoding, transcription and translation, and gene expression and additively determination of purification performance of this adsorbent(s) was aimed. Adenine and guanine incorporated poly(2-hydroxyethyl methacrylate-N-methacryloyl-adenine) [poly(HEMA-MAD)] and poly(2-hydroxyethyl methacrylate-N-methacryloyl-guanine) [poly(HEMA-MAG)] cryogels were used. Poly(HEMA-MAD) and poly(HEMA-MAG) cryogels are obtained in a partially frozen aqueous medium by copolymerization of monomers MAD and MAG with HEMA. Cryogels were characterized via FTIR,SEM, BET method, TGA, and swelling test methods. Swelling ratios of poly(HEMA-MAD),and poly(HEMA-MAG) cryogels used were found as 510% and 496%, and Maximum RNA adsorption capacities of poly(HEMA-MAD) and poly(HEMA-MAG) cryogels were found as 11.86 and 11.94 mg g, respectively.