L-FenilalaninIn Tanınmasına Yönelik Afinite Kartuşlarının Hazırlanması

Abstract

Affinity chromatography is a chromatographic method of separating biological mixtures, based on highly specific biological interactions. Hydrophobic interaction chromatography, (HIC), which is a sub-group of affinity chromatography, takes advantage of the hydrophobicity of proteins promoting its separation on the basis of hydrophobic interactions between immobilized hydrophobic ligands and nonpolar regions on the surface of the proteins. L-Phenylalanine (L-Phe) is a hydrophobic amino acid and the central nervous system requires it for normal operation. L-Phe is used in medicine, pharmaceutical and food industry. Enantiomers have the same chemical or physical properties, but different biological, pharmacological and toxicological effects due to differences such as the nature of different biological and pharmacological activity.