Prostat Kanserine Özgül Glikoproteinlerin Tanımlanması İçin Lektin Afinite Sorbentlerin Hazırlanması

Abstract

Prostate specific antigen (PSA) is a glycoprotein and has been used as a biomarker in the prognosis and diagnosis of prostate cancer (PCa). The alterations in the glycosylation feature of PSA during disease processes have brought a new and important perspective in order to understand the pathogenesis. In this study, Concanavalin A (Con A) immobilized magnetic poly(glycidyl methacrylate) (mPGMA) beads were prepared and PSA binding capacities of these beads were compared in PCa and BPH using lectin-glycoprotein interactions. For generating a magnetic property, iron oxide nanoparticles were synthesized. Then, mPGMA beads were prepared by dispersion polymerization using these iron oxide nanoparticles. Characterization studies were performed with scanning electron microscopy, electron spin resonance, thermogravimetric analysis, surface area analysis, fourier transform infrared spectroscopy and vibrating sample magnetometer.