Show simple item record

dc.contributor.authorBuyukkoroglu, Gulay
dc.contributor.authorYazan, Emine Yasemin
dc.contributor.authorOner, Ayse Filiz
dc.date.accessioned2019-12-16T10:18:18Z
dc.date.available2019-12-16T10:18:18Z
dc.date.issued2015
dc.identifier.issn1300-0527
dc.identifier.urihttps://doi.org/10.3906/kim-1412-40
dc.identifier.urihttp://hdl.handle.net/11655/20045
dc.description.abstractThe aim of this study was to prepare and evaluate stable cationic solid lipid nanoparticles (SLNs) as colloidal carriers for gene therapy. SLNs were mainly composed of three different biocompatible and biodegradable matrix lipids called tripalmitin, glyceryl dibehenate, and triglyceride, all containing the cationic lipid N-(1-(2,3-dioleoyloxy)propy1)-N, N, N-trimethylammonium (DOTAP (R)). Each of these SLNs were divided into three parts and characterized by applying different processes: Part I was autoclaved (121 degrees C, 15 min), Part II was lyophilized (5 mbar, 50 degrees C), and Part III was kept in its intact form. These parts were stored at 4 degrees C, 25 degrees C (room temp.), and 40 degrees C for short-term stability tests. The formulations were tested physically regarding the particle size and zeta potential. pUC18 plasmid DNA was used as the genetic material. Zeta potentials of all SLNs and pDNA-SLN complexes were determined to be highly positive (between +28.90 and +59.39). Crystallization processes of lipid matrices were characterized by X-ray diffractometry and differential scanning calorimetry. pDNA binding ability of SLNs and the stability of pDNA-SLN complexes with DNase I enzyme were also determined by gel electrophoresis. It was determined that all formulations became positively charged with DOTAP (R). They were able to bind DNA and were partially protective against enzyme degradation. Although additional studies are necessary, this study reveals the promising potential of this gene delivery system for gene therapy.
dc.language.isoen
dc.publisherScientific Technical Research Council Turkey-Tubitak
dc.relation.isversionof10.3906/kim-1412-40
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChemistry
dc.subjectEngineering
dc.titlePreparation and Physicochemical Characterizations of Solid Lipid Nanoparticles Containing Dotap for Dna Delivery
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalTurkish Journal Of Chemistry
dc.contributor.departmentFarmasötik Teknoloji
dc.identifier.volume39
dc.identifier.issue5
dc.identifier.startpage1012
dc.identifier.endpage1024
dc.description.indexWoS
dc.description.indexScopus


Files in this item

This item appears in the following Collection(s)

Show simple item record