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dc.contributor.authorCetin, Meltem
dc.contributor.authorYoun, Yu Seok
dc.contributor.authorCapan, Yilmaz
dc.contributor.authorLee, Kang Choon
dc.date.accessioned2019-12-16T10:18:17Z
dc.date.available2019-12-16T10:18:17Z
dc.date.issued2008
dc.identifier.issn1530-9932
dc.identifier.urihttps://doi.org/10.1208/s12249-008-9165-2
dc.identifier.urihttp://hdl.handle.net/11655/20042
dc.description.abstractThis study was performed to prepare and characterize the biotinylated Salmon calcitonin (sCT) for oral delivery and evaluate the hypocalcemic effect of biotinylated-sCTs in rats. Biotinylated sCTs was characterized by using high performance liquid chromatography ( HPLC) and MALDITOF-MS. The effect of biotinylation on permeability across Caco-2 cell monolayers was examined. Their hypocalcemic effect was determined in rats. Mono- and di-bio-sCTs were separated by reverse phase HPLC. The molecular weights of mono-bio-sCT and di-bio-sCT were determined to be 3,660.5 and 3,900.2 Da, respectively. The permeability of biotinylated-sCTs across Caco-2 cell monolayers was observed with a significant enhancement compared with sCT. Intrajejunal (ij) administration of mono-bio-sCT and di-bios-CT resulted in sustained reduction in serum calcium levels, with a maximum reduction (% max(d)) of 21.6% and 30% after 4 h and 6 h of application, respectively. The biotin conjugation of sCT may be a promising strategy for increasing the oral bioavailability of sCT and achieving sustained calcium-lowering effects.
dc.language.isoen
dc.publisherSpringer
dc.relation.isversionof10.1208/s12249-008-9165-2
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPharmacology & Pharmacy
dc.titlePreparation and Characterization of Salmon Calcitonin-Biotin Conjugates
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalAaps Pharmscitech
dc.contributor.departmentFarmasötik Teknoloji
dc.identifier.volume9
dc.identifier.issue4
dc.identifier.startpage1191
dc.identifier.endpage1197
dc.description.indexWoS


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