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dc.contributor.authorOzek, Nihal Simsek
dc.contributor.authorSara, Yildirim
dc.contributor.authorOnur, Rustu
dc.contributor.authorSevercan, Feride
dc.date.accessioned2019-12-16T10:10:03Z
dc.date.available2019-12-16T10:10:03Z
dc.date.issued2010
dc.identifier.issn0144-8463
dc.identifier.urihttps://doi.org/10.1042/BSR20080150
dc.identifier.urihttp://hdl.handle.net/11655/20018
dc.description.abstractStatins are commonly used drugs in the treatment of hypercholesterolaemia. There are many adverse effects of statins on skeletal muscle, but the underlying mechanisms remain unclear. In the present study, the effects of low dose (20 mg/kg) simvastatin, a lipophilic statin, on rat EDL muscle (extensor digitorum longus muscle) were investigated at the molecular level using FTIR (Fourier-transform infrared) spectroscopy. FTIR spectroscopy allows us rapid and sensitive determination of functional groups belonging to proteins, lipids, carbohydrates and nucleic acids simultaneously. The results revealed that simvastatin treatment induces a significant decrease in lipid, nucleic acid, protein and glycogen content. A significant increase in the lipid/protein and nucleic acid/protein ratios was also obtained with simvastatin treatment. Furthermore, an increase in lipid order and membrane fluidity was detected. A decrease in the bandwidth of the amide I band and shifting of the position of this band to higher frequency values in treated muscle indicates structural changes in proteins. Detailed secondary structure analysis of the amide I band revealed a significant increase in antiparallel and aggregated beta-sheet, random coil structure and a significant decrease in beta-sheet structure, which indicates protein denaturation.
dc.language.isoen
dc.publisherPortland Press Ltd
dc.relation.isversionof10.1042/BSR20080150
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiochemistry & Molecular Biology
dc.subjectCell Biology
dc.titleLow Dose Simvastatin Induces Compositional, Structural and Dynamic Changes in Rat Skeletal Extensor Digitorum Longus Muscle Tissue
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalBioscience Reports
dc.contributor.departmentFarmakoloji
dc.identifier.volume30
dc.identifier.issue1
dc.identifier.startpage41
dc.identifier.endpage50
dc.description.indexWoS


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