dc.contributor.author | Dikmen, E | |
dc.contributor.author | Kara, M | |
dc.contributor.author | Dikmen, G | |
dc.contributor.author | Cakmak, H | |
dc.contributor.author | Dogan, P | |
dc.date.accessioned | 2019-12-12T06:40:31Z | |
dc.date.available | 2019-12-12T06:40:31Z | |
dc.date.issued | 2003 | |
dc.identifier.issn | 1010-7940 | |
dc.identifier.uri | https://doi.org/10.1016/S1010-7940(02)00741-8 | |
dc.identifier.uri | http://hdl.handle.net/11655/16565 | |
dc.description.abstract | Objective: Definitive diagnosis of lung cancer with conventional methods may sometimes be difficult in clinical practice. Telomerase is a ribonucleoprotein DNA polymerase that maintains the telomeric region of chromosomes during successive rounds of cell division. Telomerase activity in body cavity fluids has been advocated to be a potential diagnostic marker for malignancy. We investigated the diagnostic value of telomerase activity in bronchial lavage samples of patients undergoing diagnosis of lung cancer. Methods: A total of 29 bronchial lavage samples were collected from patients in whom the diagnosis was confirmed with cytological and/or histological examinations. Patients were classified as lung cancer patients (Group 1, n = 22) and patients with benign disease (Group 2, n = 7). Telomerase activity was determined with polymerase chain reaction-based TRAP (The telomeric repeat amplification protocol) assay. Results: Cytological examination was diagnostic in 12 (54.5%) of 22 patients in Group 1, and in all seven patients of Group 2 (P = 0.063). Telomerase activity was positive in 16 (72.7%) of Group I patients, while it was positive in only 1 (14.3%) sample of a lung abscess in Group 2 (P = 0.011). The sensitivity rate of cytological examination when combined with telomerase activity (81.8%) was significantly greater than that of cytological examination alone (54.5%) (P = 0.031). The sensitivity and specificity of telomerase activity were 72.7 and 85.7%, respectively. Telomerase activity had a positive predictive value as 0.94 and negative predictive value as 0.50. Diagnostic accuracy of telomerase activity was 75.8%. Conclusion: Telomerase activity in bronchial lavage is a highly sensitive diagnostic biomarker for malignancy and a potential complementary diagnostic technique to cytological examination in the diagnosis of lung cancer. (C) 2002 Elsevier Science B.V. All rights reserved. | |
dc.language.iso | en | |
dc.publisher | Elsevier Science Bv | |
dc.relation.isversionof | 10.1016/S1010-7940(02)00741-8 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Cardiovascular System & Cardiology | |
dc.subject | Respiratory System | |
dc.subject | Surgery | |
dc.title | Detection Of Telomerase Activity In Bronchial Lavage As An Adjunct To Cytological Diagnosis In Lung Cancer | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.relation.journal | European Journal Of Cardio-Thoracic Surgery | |
dc.contributor.department | Göğüs Cerrahisi | |
dc.identifier.volume | 23 | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | 194 | |
dc.identifier.endpage | 199 | |
dc.description.index | WoS | |
dc.description.index | Scopus | |