dc.contributor.author | Rifat, Dalin | |
dc.contributor.author | Campodnico, Victoria L. | |
dc.contributor.author | Tao, Jing | |
dc.contributor.author | Miller, James A. | |
dc.contributor.author | Alp, Alpaslan | |
dc.contributor.author | Yao, Yufeng | |
dc.contributor.author | Karakousis, Petros C. | |
dc.date.accessioned | 2019-12-12T06:24:58Z | |
dc.date.available | 2019-12-12T06:24:58Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1746-0913 | |
dc.identifier.uri | https://doi.org/10.2217/fmb-2017-0022 | |
dc.identifier.uri | http://hdl.handle.net/11655/16227 | |
dc.description.abstract | Aim: There is controversy regarding the potential fitness costs of rifampicin (RIF) resistance-conferring mutations in the Mycobacterium tuberculosis (Mtb) rpoB gene. We characterized the pathogenicity of an Mtb RpoB H526D mutant. Materials & methods: A mutant containing the RpoB H526D mutation was isolated from wild-type Mtb grown on RIF-containing plates and complemented for determination of in vitro and in vivo fitness costs. Results: The RpoB H526D mutant showed reduced survival relative to control strains during progressive hypoxia and delayed growth following resuscitation from nutrient starvation (p < 0.05), which was associated with reduced expression of the resuscitation-promoting factor genes rpfB, rpfC and rpfE. Relative to the isogenic wild-type strain, the mutant showed significantly attenuated growth and long-term survival as well as reduced inflammation in mouse lungs. Conclusion & future perspective: Our data suggest that RpoB H526D mutation confers a fitness cost during growth-limiting conditions in vitro and in mouse lungs. | |
dc.language.iso | en | |
dc.publisher | Future Medicine Ltd | |
dc.relation.isversionof | 10.2217/fmb-2017-0022 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Microbiology | |
dc.title | In Vitro And In Vivo Fitness Costs Associated With Mycobacterium Tuberculosis Rpob Mutation H526D | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.relation.journal | Future Microbiology | |
dc.contributor.department | Tıbbi Mikrobiyoloji | |
dc.identifier.volume | 12 | |
dc.identifier.issue | 9 | |
dc.identifier.startpage | 753 | |
dc.identifier.endpage | 765 | |
dc.description.index | WoS | |