Titanyum Alaşım Yüzeyleri Selektif Lazer Ergitme İşlemi ile Kaplanması Sonrası Hgf-1 Proliferasyonunun Değerlendirilmesi
Özet
Titanium and its alloys are the most common dental implant material currently. For the best bone-implant contact, machined titanium is subjected to various surface treatments. In this study, aiming to improve both biocompatibility and surface characterictics, human gingival fibroblast proliferation were evaluated after covering machined Grade 5 titanium discswith Grade 23 titanium by using SLM technology. For this purpose, 5-mm-diameter 48 titanum Grade 5 specimen was divided into four groups (n=12). The first group wasn't subjected to any surface treatment. SLA procedure was applied to Group 2.The surfaces of the specimens in Group 3 and Group 4 were covered Grade 23 titanium by SLM process. After this step, only Group 4 was applied to SLA procedure.Th surface topographyof the specimens were evaluated with SEM and optic profilometer.HGF-1 cell culture was performed following specimen sterilization by UV (n=8). After 24,48 and 72 hours, HGF-1 viability on the surface was calculated with MTT assay. Results showed that SLM process increased the surface roughness. The most stable and the vitality ratio was measured on the SLA group (Group 2). The following gropu was Group 4 that both SLM and SLA procedure were applied. Control group had the lowest cell viability for all test results. Grup 3 couldn't showed the stable results because the metal oxide layer could'nt be eliminated from the surface. Grade 23 titanium-coated surfaces by using SLM process has shown promising results when the surfaces cleaned with strong acids and sandblasting.
