Entomopatojen Fungusların Sentezlediği Beauverisinin Bazı Tarım Zararlıları Üzerindeki Etkisinin Araştırılması

Abstract

In this study, a molecularly imprinted cryogel column was used to purify beauvericin from fungal sources. The aim of this study was to purify beauvericin synthesized from 3 different B. bassiana (Lül1, KVL03129, Ank12) and 3 different P. fumosoroseus (Bey9, 0614, Bb12) strains with a molecularly imprinted cryogel column and to test the entomopathogenic diverse effect of beauvericin on the larvae of Galleria mellonella, Tenebrio molitor and Leptinotarsa decemlineata with different application methods at different concentrations (0.001M, 0.0001M, 0.0000 M, and 0.000001 M BEA) of beauvericin. In the study, beauvericin synthesized from 3 different B. bassiana and 3 different P. fumosoroseus strains were purified using a molecularly imprinted cryogel column. It was found that the maximum adsorption of BEA from the beauvericin-imprinted cryogel column occurred in MES buffer pH 6.5, at 25°C, at a flow rate of 1 mL/min. Beauvericin purified from six different fungal sources and commercial BEA were tested on G. mellonella and T. molitor larvae by injection and spraying methods. It was found that Ank12-BEA applied by injection and spraying methods on G. mellonella larvae killed half of the insect population in 2.370 days, and 2.819 days, respectively. KVL 03129-BEA applied on T. molitor larvae using injection method and spraying methods killed half of the insect population in 1.904 days, and 4.918 days, respectively. Beauvericin purified from six different fungal sources and commercial BEA were tested on L. decemlineata larvae by injection, spraying, and leaf dipping methods. It was found that 0614-BEA applied by injection, spraying and leaf dipping methods on L. decemlineata larvae killed half of the insect population in 8.053 days, 9.160 days, and 10.831 days, respectively. Beauvericin purified from six different fungal sources was found to be successful on insects at all application methods and all beauvericin concentrations. According to LT50 values, the highest BEA concentration was found to be the most effective. Based on the lethal dose values on the 5th, 8th, and 10th days, it was found that as the exposure time of insects to BEA increased, the dose of BEA needed to kill half the insect population decreased.