Ürik Asit Tayini İçin Moleküler Baskılanmış Monolitik Kolonların Hazırlanması

Abstract

Uric acid is a heterocyclic organic compound that is the end product of purine metabolism in humans and great apes. In humans, UA is an end product of the catabolism of purine nucleotides of endogenous origin and exogenous (dietary purines). When the serum level exceeds 7.0 mg/dL, it crystallizes and precipitates in soft tissue, joints, and kidneys. Uric acid in abnormal concentrations in the blood and urine has been associated with health problems such as gout arthritis, hyperuricemia, hypertension, pneumonia and kidney damage. Monoliths are very porous stationary phases. Monoliths are prepared from monomeric precursors, with polymerization of the solvent mixture, a skeletal structure with interconnected pores is formed. Monoliths have two different pore size distributions, macropores in the micrometer order and mesopores in the nanometer order, which provide high surface area. Due to their extremely fast mass transfer kinetics, monolithic structures have low mass transfer resistance. Molecularly imprinted polymers, MIPs, are best described as synthetic analogs of natural biological antibody antigen systems. Hence, they work with a "lock and key" mechanism to selectively bind the molecule they are tuned to during manufacture. MIPs offer the specificity and selectivity of biological receptors advantageously in terms of low cost and environmental resistance. In this thesis, uric acid molecule was imprinted on PHEMA monoliths using molecular imprinting technology. In molecular imprinting process, metal-chelate monomer was prepared by interacting MAH functional monomer with Cu(II) metal ion and then pre-complex was prepared by interacting with uric acid. Characterization studies were carried out for the prepared pre-complex and uric acid imprinted PHEMA monoliths. Adsorption-desorption experiments of UA imprinted PHEMA monoliths were studied from aqueous solutions of uric acid. The MIP PHEMA monolith was optimized by studying parameters such as column flow rate, increased uric acid concentration, temperature and adsorption rate. To demonstrate the selectivity of the uric acid imprinted PHEMA monolith, competitive adsorption experiments were carried out in aqueous solution in a continuous system. Selectivity comparisons for uric acid were made with creatinine and ascorbic acid molecules.