Penisilin Açilaz (Pa) Saflaştırılması İçin Süpermakrogözenekli Hidrofobik Afinite Sorbentler

Abstract

PA (PA, EC 3.5.1.11) is an important enzyme in drug industry. PA is used in the production of 6-aminopenicillanic acid (6-APA) which is used as a raw material in the production of semi synthetic penicillins. Although there are many methods for PA purification, affinity chromatography is advantageous as it provides efficient one step purification. In this study, poly(2-hydroxyethyl methacrylate) based cryogel column containing hydrophobic N-methacryloyl-L-tryptophan (MATrp) functional monomer as a ligand was prepared. Interaction of MATrp with amino acids in PA structure is the basis of hydrophobic interaction chromatography in this study.