Zoledronik Asit Modifiye Stronsiyum/Hidroksiapatit Nanopartikül İçeren Hidrojellerin Geliştirilmesi

Abstract

Within the scope of this doctoral thesis, new types of nanocomposite hydrogels consisting of zoledronic acid/strontium hydroxyapatite nanoparticles and carboxymethyl chitosan/oxidised dextran (CMC/OD) hydrogels were studied. Pure hydroxyapatite, 5%, 10% and 15% (w/w) strontium substituted strontium hydroxyapatite nanoparticles were produced and modified with zoledronic acid at ratios of 5% to 7,5% (w/w). The modified strontium hydroxyapatite nanoparticles were incorporated into CMC/OD hydrogels. Zoledronic acid modified strontium hydroxyapatite nanoparticles were characterised by Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Spectroscopy (EDS) and X-Ray Diffraction (XRD). The chemical properties of CMC/OD hydrogels were investigated by Fourier Transform Infrared Analysis (FTIR) and SEM analysis. The physical properties of CMC/OD hydrogels were determined by degradation behaviour and rheological measurements. Cell-material interactions were investigated in vitro. The results showed that the incorporation of hydroxyapatite nanoparticles into CMC/OD hydrogels would significantly improve the rheological properties. The addition of strontium to hydroxyapatite nanoparticles significantly enhanced cell proliferation. A significant increase in alkaline phosphatase (ALP) and calcium accumulation was observed with zoledronic acid modification. In conclusion, CMC/OD nanocomposite hydrogels containing zoledronic acid modified strontium hydroxyapatite show potential in orthopaedic and craniofacial applications due to their superior properties such as the ability to be easily injected into targeted areas, a strong antibacterial activity that prevents infections, and self healing capabilities that promote tissue regeneration and repair.