Bazı Plantago ekstrelerinin antimutajenik etkilerinin Salmonella/Escherichia coli WP2 test sistemiyle belirlenmesi

Abstract

Human beings encounter several natural or artificial environmental agents throughout their lives. In the protection against the detrimental effects of these agents that might endanger human health more or less, or in the extermination of such effects, some plants and secondary metabolites come to the fore because of their antimutagenic potential. Plantago species are known to be rich in secondary metabolites. In this study, antimutagenic effects of methanol extracts that were obtained from the aerial parts of five types/subtypes that belong to Plantago species that grow in Turkey (Plantago major subsp. Plantago intermedia, P.major major subsp. major, Plantago scabra, Plantago holosteum, Plantago lagopus) were analysed with Ames test system and E.coli WP2 test system. The study has been carried out with the use of Salmonella typhimurium TA 98, TA100 and TA 102 strains in the Ames test system, and with the use of Escherichia coli WP2 uvr A strain in the E.coli WP2 test system. When the results obtained within the study were considered together, it was observed that the plant type that had maximum antimutagenic activity was Plantago major subsp. major that showed antimutagenic activity at varying rates in all concentrations that were applied on TA 102, TA 100 and E.coli WP2 strains. Especially the effects of Plantago major subsp. intermedia (48 % inhibition, 400 µg/plate concentration) and Plantago major subsp. major (47 % inhibition, 100 µg/plate concentration; 45 % inhibition, 25 µg/plate concentration) extracts on the E.coli WP2 strain were remarkable. It was also determined that the extract obtained from Plantago scabra type had moderate antimutagenic activity in all concentrations applied on TA 100 strain (39 % maximum inhibition, 100 µg/plate concentration). While Plantago lagopus extract showed weak antimutagenic activity for all Salmonella typhimurium strains in 400 µg/plate concentration, 29 % inhibition was obtained in the Escherichia coli WP2 uvr A strain at this dose. It was observed that Plantago holosteum extract showed weak antimutagenic activity in all strains. In conclusion, it was determined that species /subspecies that belong to Plantago carried antimutagenic activity, and further study could be suggested in terms of various parameters.