Bazı Gram Negatif Bakterilerde Kontakt Bağımlı İnhibisyon Mekanizmasının Araştırılması

Abstract

Examining the interactions of microorganisms with each other provides us with the opportunity to get to know microorganisms better and find clues on how to deal with them. The discovery of antibiotics, biofilm formation and mechanisms such as quorum sensing emerged as a result of investigating these interactions. Due to the resistance mechanisms developed by microorganisms, the effect of existing methods decreases in our fight against microorganisms, and this makes it necessary to investigate new mechanisms. CDI (Contact Dependent Growth Inhibition) mechanism emerges as a mechanism by which bacteria inhibit each other, especially in competition within the species. After bacteria with CDI mechanism bind to the receptors of bacteria that do not have the CDI mechanism, they release toxins into them, and this toxin toxin causes a bacteriostatic effect in the target bacteria. In this context, the aim of the presented thesis is to elucidate the CDI mechanisms of Protues and Salmonella species. In this thesis, various Proteus and Salmonella species, both from the American Cultural Collection and our own isolates, were studied. It was determined that the RefSeq: WP_094959879.1 protein belonging to the Proteus mirabilis strain registered in NCBI with the number ASM226535v1 could be CdiB, the protein numbered WP_094959878.1 could be CdiA and the protein numbered WP_063108584.1 could be the CdiI protein. CDI negative Proteus mirabilis ATCC® 43071 Δcdi BAI :: ampR mutant strain was obtained from Proteus mirabilis ATCC® 43071 strain by homologous recombination method and the contact-dependent inhibition mechanism of this strain was elucidated with various parameters.