dc.contributor.author | Gökaltun, Aslıhan | |
dc.contributor.author | Kang, Young Bok (Abraham) | |
dc.contributor.author | Yarmush, Martin L. | |
dc.contributor.author | Usta, O. Berk | |
dc.contributor.author | Asatekin, Ayse | |
dc.date.accessioned | 2021-06-08T05:23:39Z | |
dc.date.available | 2021-06-08T05:23:39Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://dx.doi.org/10.1038/s41598-019-43625-5 | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517421/ | |
dc.identifier.uri | http://hdl.handle.net/11655/24648 | |
dc.description.abstract | Poly(dimethylsiloxane) (PDMS) is likely the most popular material for microfluidic devices in lab-on-a-chip and other biomedical applications. However, the hydrophobicity of PDMS leads to non-specific adsorption of proteins and other molecules such as therapeutic drugs, limiting its broader use. Here, we introduce a simple method for preparing PDMS materials to improve hydrophilicity and decrease non-specific protein adsorption while retaining cellular biocompatibility, transparency, and good mechanical properties without the need for any post-cure surface treatment. This approach utilizes smart copolymers comprised of poly(ethylene glycol) (PEG) and PDMS segments (PDMS-PEG) that, when blended with PDMS during device manufacture, spontaneously segregate to surfaces in contact with aqueous solutions and reduce the hydrophobicity without any added manufacturing steps. PDMS-PEG-modified PDMS samples showed contact angles as low as 23.6° ± 1° and retained this hydrophilicity for at least twenty months. Their improved wettability was confirmed using capillary flow experiments. Modified devices exhibited considerably reduced non-specific adsorption of albumin, lysozyme, and immunoglobulin G. The modified PDMS was biocompatible, displaying no adverse effects when used in a simple liver-on-a-chip model using primary rat hepatocytes. This PDMS modification method can be further applied in analytical separations, biosensing, cell studies, and drug-related studies. | |
dc.language.iso | en | |
dc.relation.isversionof | 10.1038/s41598-019-43625-5 | |
dc.rights | Attribution 4.0 United States | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Simple Surface Modification Of Poly(Dimethylsiloxane) Via Surface Segregating Smart Polymers For Biomicrofluidics | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.relation.journal | Scientific Reports | |
dc.contributor.department | Kimya Mühendisliği | |
dc.identifier.volume | 9 | |
dc.description.index | PubMed | |
dc.description.index | WoS | |
dc.description.index | Scopus | |