Doku Rejenerasyonunda Kullanılmak Üzere Etken Madde Yüklü Nanofibriler Yapıların Hazırlanması ve Karakterizasyonu

Abstract

In this study PHBV nanofibrillar structures were fabricated from different concentrations of PHBV solutions (10%, 13% and 15% (w/v)) by electrospinning and electrospinning parameters were optimized. Nanofibrillar structures with the most smooth and homogeneous morfologies were obtained at 10% (w/v) PHBV concentration. Curcumin (Cur), which is known with antimicrobial, anti-inflammatory, wound-healing properties and isolated from Curcuma longa L., was loaded in different concentrations (0.1%, 0.3% and %0.5% (w/v)) to the PHBV solutions prepared at this concentration. The characterization of PHBV and curcumin loaded PHBV nanofibrillar structures were performed by Scanning Electron Microscope(SEM), Fourier Transform Infrared Spectroscopy(ATR-FTIR), X-ray Diffraction(XRD) and Differential Scanning Calorimeter(DSC). The SEM analyses revealed that the fiber diameter of these nanofibrillar structures varied in the range of 50-838 nm. Mechanical testing machine was used to determine the mechanical properties. Cytotoxicity evaluation was investigated by MTT test. Indirect cytotoxicity results showed that PHBV and curcumin loaded PHBV nanofiber extracts aren't toxic to L929 mouse fibroblasts. The adhesion and proliferation results of L929 fibroblasts indicated that the best and effective adhesion were observed for 10% (w/v) PHBV and 0.5% (w/v) curcumin loaded PHBV nanofibrillar structures. In vitro curcumin release characteristic from curcumin loaded PHBV nanofibrillar structures was examined. The degradation profiles of these nanofibrillar structures, which were kept in PBS solutions containing lysozyme, were obtained based on mass reductions during 14 days. Additionally, swelling studies for these nanofibrillar structures were performed. Curcumin loaded PHBV nanofibrillar structures is expected to have great potential as a wound-burn dressing material in tissue engineering.