Nanoteknolojik Yaklaşımlarla Koagülatif Sistemlerin Geliştirilmesi

Abstract

Uncontrolled bleeding is the major cause of death in cases like war and disaster in addition of bleeding dependant trauma encountered in daily life. Effective bleeding control is essential because a lot of injured patients or limited medical equipment on battlefield. Currently for bleeding control was used such as bandage, direct pressure, tourniquet or cauterization. But these conventional techniques remain incapable or not used in much injury. Therefore hemostatic agent requirement, that is portable, low cost, no adverse effects to healing, effective control of bleeding, easy administration even by layperson or by first-aider, increases day by day. When the conditions and environmental factors play a role in hemostasis were considered, products produced by nanotechnological approaches will iv facilitate obviously the hemostasis. Similarity of the nanofibrilar structure to collagen/elastin fibers in extracellular matrix and its high surface area/volume ratio will accelerate thrombocyte migration in structure. Within the scope of the thesis; it’s planned that the double layer blood stopping patches are prepared. At first step, top layer of hemostatic patches are prepared from cellulose and chitosan that is frequently used in medical industry. Thereafter, hemostatic agents (calcium ions, vitamin K, kaolin etc.) that plays active role in coagulation cascade are incorporated the structure of the hemostatic patches. A nanofibrillar layer membrane on the top is created from the silk fibroin that widely used as a suture material and phosphatdylcholine that be in the structure of cell. The performances of the prepared modified silk fibroin nanofibrillar patches are investigated comparatively. Surface properties of hemostatic dressing are characterized using Scanning Electron Microscopy (SEM). The activity of the prepared hemostatic dressings is examined by in-vitro and in-vivo coagulation tests. The coagulation time of blood is determined using by partial thromboplastin time (aPTT) and protrombin time (PTZ) tests. The amount of adhered thrombocyte on the hemostatic dressings is compared using LDH assay. In vivo study, the hemostatic activity of the hemostatic dressings is tested in healty and diabetic rat femoral artery model.