dc.contributor.author | Çelebi, B. | |
dc.date.accessioned | 2020-01-17T07:14:03Z | |
dc.date.available | 2020-01-17T07:14:03Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1233-2356 | |
dc.identifier.uri | https://doi.org/10.1556/1326.2017.29.2.00 | |
dc.identifier.uri | http://hdl.handle.net/11655/21729 | |
dc.description.abstract | New polyacrylate-based monosized-porous polymer beads were proposed as a stationary phase for the separation of polar compounds by microbore reversed-phase chromatography. For this purpose, monosized-porous poly(glycerol dimethacrylate-co-glycerol-1,3-diglycerolate diacrylate), poly(GDMA-co-GDGDA), beads with hydroxyl functionality were synthesized by a modified seeded polymerization. The selected octa-decylating agent, stearoyl chloride (SC), was covalently attached onto the hydrophilic beads via a direct, single stage reaction with a simple synthetic route. SC attached-poly(GDMA-co-GDGDA) beads were slurry-packed into the microbore columns and used as separation medium microbore reversed-phase chromatography. The stationary phase was used for separation of alkylbenzenes and polar analytes by micro reversed-phase chromatography, using mobile phases with low acetonitrile content. Theoretical plate number (TPN) values up to 12,000 plates m(-1) and 10,000 plates m-1 for alkylbenzenes and polar analytes, respectively, were achieved. The results also showed that poly(GDMA-co-GDGDA) hydrogel beads are a promising starting material for a number of chromatographic applications like reversed-phase (RP) chromatography, hydrophilic interaction chromatography (HILIC), ion-exchange chromatography (IEC), and affinity chromatography with a single-stage surface modification. | tr_TR |
dc.language.iso | en | tr_TR |
dc.publisher | Akademiai Kiado Rt | tr_TR |
dc.relation.isversionof | 10.1556/1326.2017.29.2.00 | tr_TR |
dc.rights | info:eu-repo/semantics/openAccess | tr_TR |
dc.subject | Chemistry | tr_TR |
dc.subject | Reversed-phase chromatography | tr_TR |
dc.subject | Stearoyl chloride | tr_TR |
dc.subject | Hydrogel beads | tr_TR |
dc.subject | Microbore columns | tr_TR |
dc.subject | Polar analytes | tr_TR |
dc.subject.lcsh | Kimya | tr_TR |
dc.title | A Simple Synthetic Route For The Preparation of A Reversed-Phase Stationary Phase Based on Monosized-Porous Hydrogel Beads And Its Chromatographic Use For Separation Of Small Molecules | tr_TR |
dc.type | info:eu-repo/semantics/article | tr_TR |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.relation.journal | Acta Chromatographica | tr_TR |
dc.contributor.department | Kök Hücre Bilimleri | tr_TR |
dc.identifier.volume | 29 | tr_TR |
dc.identifier.issue | 2 | tr_TR |
dc.identifier.startpage | 143 | tr_TR |
dc.identifier.endpage | 159 | tr_TR |
dc.description.index | WoS | tr_TR |
dc.description.index | Scopus | tr_TR |
dc.funding | Yok | tr_TR |