Lipazın Trichoderma Citrinoviride Den Üretimi ve Enzimin Bazı Kinetik Özelliklerinin Saptanması

Abstract

In this study, the production of lipase is aimed from a new fungal source that was isolated from soil. Fungi was isolated from soil locality Kocaeli, İlimtepe. Subsequently it was determinated as Trichoderma citrinoviride by the analysis of 18S rRNA sequence. This microorganism have been used as a source of lipase. In the production of lipase, culture conditions and media components are investigated as important parameters. Various carbon and nitrogen sources are used for lipase activity investigation. For production of lipase glucose was found to be most suitable carbon source and peptone was found to be most suitable nitrogen source.