Nevşehir – Kapadokya Bölgesi Nde Kara Kaplumbağalarındaki (Testudo Graeca, Lınnaeus 1758) Bazı Enzim Ve Hormon Düzeylerinin Araştırılması

Abstract

This study was on common tortoise (Testudo graeca) (55 tortoises from Kaplumbaga valley and 34 tortoises from Nar valley) between April to October 2012 in Nevsehir, Cappadocia Region. The enzyme and hormone levels are investigated by taking the blood samples. Enzymes, esterase (cholinesterases (BChE) carboxylesterases (CbE)) and glutathione enzymes (glutathione S transferases (GST), glutathione reductase (GR), glutathione peroxidase (GPx)) and sex steroid hormones (Testosterone and Estradiol) are considered as parameters. The tortoise blood samples were compared in two areas by seasonal fluctation. BChE enzyme activities were significantly higher in Kaplumbaga valley (516,56 411,26 nmol min ml serum) than Nar Valley (333,86 180,94 nmol min ml serum) in June 2012 with both acetylthiocholine iodide and butyrlthiocholine iodide substrates. CbE activities were tested by 4 different substrates 1-Naphytlacetate:1-NA, 1-Naptylbutyrate:1-NB, 4-Nitrophenylacetate :4-NPA, 4-Nitrophenylbuty