Basit öğe kaydını göster

dc.contributor.authorTacal, Ozden
dc.contributor.authorLockridge, Oksana
dc.date.accessioned2019-12-16T10:29:25Z
dc.date.available2019-12-16T10:29:25Z
dc.date.issued2010
dc.identifier.issn0260-437X
dc.identifier.urihttps://doi.org/10.1002/jat.1518
dc.identifier.urihttp://hdl.handle.net/11655/20118
dc.description.abstractOrganophosphorus pesticides used most commonly in Turkey include methamidophos, dichlorvos, O-methoate and diazinon. These toxic chemicals or their metabolites make a covalent bond with the active site serine of butyrylcholinesterase. Our goal was to identify the adducts that result from the reaction of human butyrylcholinesterase with these pesticides. Highly purified human butyrylcholinesterase was treated with a 20-fold molar excess of pesticide. The protein was denatured by boiling and digested with trypsin. MS and MSMS spectra of HPLC-purified peptides were acquired on a MALDI-TOF-TOF 4800 mass spectrometer. It was found that methamidophos added a mass of +93, consistent with addition of methoxy aminophosphate. A minor amount of adduct with an added mass of +109 was also found. Dichlorvos and O-methoate both made dimethoxyphosphate (+108) and monomethoxyphosphate adducts (+94). Diazinon gave a novel adduct with an added mass of +152 consistent with diethoxythiophosphate. Inhibition of enzyme activity in the presence of diazinon developed slowly (15 h), concomitant with isomerization of diazinon via a thiono-thiolo rearrangement. The isomer of diazinon yielded diethoxyphosphate and monoethoxyphosphate adducts with added masses of +136 and +108. MSMS spectra confirmed that each of the pesticides studied made a covalent bond with serine 198 of butyrylcholinesterase. These results can be used to identify the class of pesticides to which a patient was exposed. Copyright (C) 2010 John Wiley & Sons, Ltd.
dc.language.isoen
dc.publisherWiley
dc.relation.isversionof10.1002/jat.1518
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectToxicology
dc.titleMethamidophos, Dichlorvos, O-Methoate And Diazinon Pesticides Used In Turkey Make A Covalent Bond With Butyrylcholinesterase Detected By Mass Spectrometry
dc.typeinfo:eu-repo/semantics/article
dc.relation.journalJournal Of Applied Toxicology
dc.contributor.departmentBiyokimya
dc.identifier.volume30
dc.identifier.issue5
dc.identifier.startpage469
dc.identifier.endpage475
dc.description.indexWoS


Bu öğenin dosyaları:

Bu öğe aşağıdaki koleksiyon(lar)da görünmektedir.

Basit öğe kaydını göster