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dc.contributor.authorUçaktürk, Ebru
dc.date.accessioned2019-12-16T10:29:18Z
dc.date.available2019-12-16T10:29:18Z
dc.date.issued2015
dc.identifier.issn2090-8865
dc.identifier.urihttps://doi.org/10.1155/2015/707414
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670650/
dc.identifier.urihttp://hdl.handle.net/11655/20085
dc.description.abstractA sensitive and selective gas chromatography-mass spectrometry (GC-MS) method was developed and fully validated for the determination of vildagliptin (VIL) in pharmaceutical formulation. Prior to GC-MS analysis, VIL was efficiently derivatized with MSTFA/NH4I/β-mercaptoethanol at 60°C for 30 min. The obtained O-TMS derivative of VIL was detected by selected ion monitoring mode using the diagnostic ions m/z 223 and 252. Nandrolone was chosen as internal standard. The GC-MS method was fully validated by the following validation parameters: limit of detection (LOD) and quantitation (LOQ), linearity, precision, accuracy, specificity, stability, robustness, and ruggedness. LOD and LOQ were found to be 1.5 and 3.5 ng mL−1, respectively. The GC-MS method is linear in the range of 3.5–300 ng mL−1. The intra- and interday precision values were less than ≤3.62%. The intra- and interday accuracy values were found in the range of −0.26–2.06%. Finally, the GC-MS method was successfully applied to determine VIL in pharmaceutical formulation.
dc.relation.isversionof10.1155/2015/707414
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleDevelopment of Sensitive And Specific Analysis of Vildagliptin In Pharmaceutical Formulation By Gas Chromatography-Mass Spectrometry
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalJournal of Analytical Methods in Chemistry
dc.contributor.departmentAnalitik Kimya
dc.identifier.volume2015
dc.description.indexPubMed
dc.description.indexWoS
dc.description.indexScopus


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