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dc.contributor.authorZeybek, Derya Koyuncu
dc.contributor.authorZeybek, Bulent
dc.contributor.authorPekmez, Nuran Ozcicek
dc.contributor.authorPekyardimci, Sule
dc.contributor.authorKilic, Esma
dc.date.accessioned2019-12-16T09:18:46Z
dc.date.available2019-12-16T09:18:46Z
dc.date.issued2012
dc.identifier.issn0103-5053
dc.identifier.urihttps://doi.org/
dc.identifier.urihttp://hdl.handle.net/11655/19613
dc.description.abstractA polymer-based amperometric enzyme electrode (Pt/PoPD/ChOx) was prepared for the amperometric detection of free cholesterol. Firstly, poly(o-phenylenediamine) (PoPD) polymer film was prepared in acetonitrile-water medium containing o-phenylenediamine (oPD) monomer and (+/-)-10-camphorsulfonic acid (HCSA) on Pt electrode by the use of cyclic voltammetry technique. Cholesterol oxidase (ChOx) enzyme was immobilized onto Pt/PoPD electrode surface. The determination of cholesterol was performed via monitoring of the oxidation current of enzymatically produced H2O2 at + 0.7 V vs. Ag/AgCl. Optimum buffer concentration, pH and working temperature were found as 0.05 mol L-1, 7.5 and 40 degrees C, respectively. The working range of enzyme electrode to cholesterol was 9.8 x 10(-3)-11 mu mol L-1 and response time 150 s. The effects of possible interferences present in serum samples on response of enzyme electrode were examined. The determination of total cholesterol in serum samples was performed by using proposed Pt/PoPD/ChOx enzyme electrode and results were in good agreement with those obtained by spectrophotometric method.
dc.language.isoen
dc.publisherSoc Brasileira Quimica
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChemistry
dc.titleDevelopment Of An Amperometric Enzyme Electrode Based On Poly(O-Phenylenediamine) For The Determination Of Total Cholesterol In Serum
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalJournal Of The Brazilian Chemical Society
dc.contributor.departmentKimya
dc.identifier.volume23
dc.identifier.issue12
dc.identifier.startpage2222
dc.identifier.endpage2231
dc.description.indexWoS
dc.description.indexScopus


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