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dc.contributor.authorYilmaz, Zeliha
dc.contributor.authorDogan, A. Lale
dc.contributor.authorOzdemir, H. Ozgur
dc.contributor.authorSerper, Ahmet
dc.date.accessioned2019-12-16T06:34:46Z
dc.date.available2019-12-16T06:34:46Z
dc.date.issued2012
dc.identifier.issn0287-4547
dc.identifier.urihttps://doi.org/10.4012/dmj.2012-172
dc.identifier.urihttp://hdl.handle.net/11655/19066
dc.description.abstractThe purpose of this study was to evaluate the cytotoxic effects of commercially available root canal sealers [Sealite Ultra (SU), Tubli-Seal (TS), Tubli-Seal EWT (TS-EWT), Pulp Canal Sealer (PCS), Pulp Canal Sealer EWT (PCS-EWT), Endomethasone N (En N), and Apexit Plus (AP)] on L929 cells by using MTT assay. After incubation with each sealer's extract at 37 degrees C in a humidified air atmosphere containing 5% CO2 for 24 h, MTT (5 mg/mL) in saline was added into each well and further incubated at 37 degrees C for 4 h. Formazan precipitate was dissolved in a buffer containing 23% sodium dodecyl sulfate and 50% N,N-dimethylformamide (pH 4.7). Optical densities of dissolved formazan were read using a microplate spectrophotometer. AP, TS, and TS-EWT showed no cytotoxicity at any dilution tested. Other sealers exhibited some degree of cytotoxicity at the 1/4 and 1/2 dilutions. PCS-EWT and SD exerted more potent cytotoxicity at 1/2 dilution than the other sealers.
dc.language.isoen
dc.publisherJapanese Soc Dental Materials Devices
dc.relation.isversionof10.4012/dmj.2012-172
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDentistry
dc.subjectMaterials Science
dc.subjectOral Surgery & Medicine
dc.titleEvaluation Of The Cytotoxicity Of Different Root Canal Sealers On L929 Cell Line By Mit Assay
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalDental Materials Journal
dc.contributor.departmentEndodonti
dc.identifier.volume31
dc.identifier.issue6
dc.identifier.startpage1028
dc.identifier.endpage1032
dc.description.indexWoS
dc.description.indexScopus


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