dc.contributor.author | Tuney, Ipek | |
dc.contributor.author | Altay, Aylin | |
dc.contributor.author | Ergunay, Koray | |
dc.contributor.author | Onder, Sevgen Celik | |
dc.contributor.author | Usubutun, Alp | |
dc.contributor.author | Salman, Mehmet Coskun | |
dc.contributor.author | Bozdayi, Gulendam | |
dc.contributor.author | Karabulut, Erdem | |
dc.contributor.author | Badur, Osman Selim | |
dc.contributor.author | Yuce, Kunter | |
dc.contributor.author | Pinar, Ahmet | |
dc.date.accessioned | 2019-12-12T06:42:02Z | |
dc.date.available | 2019-12-12T06:42:02Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1300-0144 | |
dc.identifier.uri | https://doi.org/10.3906/sag-1508-155 | |
dc.identifier.uri | http://hdl.handle.net/11655/16695 | |
dc.description.abstract | Background/aim: Human papillomaviruses have been established as a risk factor for invasive carcinoma of the uterine cervix. HPV E6/E7 oncogene expression has recently emerged as a promising biomarker to determine the risk for progression to high-grade cervical lesions. The aim of this study was to evaluate HPV mRNA and DNA detection in samples with abnormal cytology. Materials and methods: Cervical specimens were obtained at the Department of Obstetrics and Gynecology via cervical brushes during January-October 2011. Liquid-based cytology slides were evaluated according to the 2001 Bethesda System. Cytology specimens from a total of 81 women with abnormal cytology were included. Real-time PCR and NASBA assays were performed to detect HPV DNA and E6/E7 mRNA, respectively. Results: HPV DNA was identified in 73 samples (90.1%). HPV E6/E7 mRNA expression was observed in 45 samples (55.6%). A statistically significant difference was observed among cytological diagnosis groups. In 25 patients, a biopsy was performed during the follow-up. HPV DNA was detected in all of these patients. HPV E6/E7 expression was present only in CIN I-III diagnosed patients. Conclusion: The E6/E7 mRNA test is a robust indicator of cytological atypia and correlates better with progressive lesions than DNA assays. | |
dc.language.iso | en | |
dc.publisher | Tubitak Scientific & Technical Research Council Turkey | |
dc.relation.isversionof | 10.3906/sag-1508-155 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | General & Internal Medicine | |
dc.title | Hpv Types And E6/E7 Mrna Expression In Cervical Samples From Turkish Women With Abnormal Cytology In Ankara, Turkey | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.relation.journal | Turkish Journal Of Medical Sciences | |
dc.contributor.department | Tıbbi Patoloji | |
dc.identifier.volume | 47 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 194 | |
dc.identifier.endpage | 200 | |
dc.description.index | WoS | |
dc.description.index | Scopus | |