Renin-Angiotensin System (Ras) Expressions in Myeloid Leukemic Cell Lines
Tarih
2013Yazar
Uz, Burak
Tatonyan, Suzin C.
Sayitoglu, Muge
Erbilgin, Yucel
Hatirnaz Ng, Ozden
Buyukasik, Yahya
Aksu, Salih
Sayinalp, Nilgun
Goker, Hakan
Ozcebe, Osman I.
Ozbek, Ugur
Haznedaroglu, Ibrahim C.
Üst veri
Tüm öğe kaydını gösterÖzet
The aim of this study is to search critical renin-angiotensin system (RAS) elements in myeloid leukemic cell lines. Human acute myeloid leukemia (AML) cell lines, KG-1 and HL-60, were cultured. We searched for the gene expression of the major RAS components in KG-1 and HL-60 cell lines by quantitative real-time polymerase chain reaction analysis (qRT-PCR). Angiotensin-converting enzyme I (ACE I) and ACE II mRNA expressions were detected in KG-1 AML cell line. Relative expression of ACE I was higher than ACE II expression in this myeloid leukemic cells. Likewise, RENIN, angiotensinogen (ANGTS), and ACE I mRNA expressions were detected in HL-60 promyelocytic cell line. Relative RENIN expression was the highest, whereas ACE I expression was the lowest in HL-60 neoplastic myeloid cells. These findings indicate that there is a biologically active local RAS in the hematopoietic system in normal and pathological states.