Kimyasal Olarak İndüklenmiş Meme Kanseri Modelinde Klorofilin-Cu Kompleksinin Antioksidan Etkinliğinin İncelenmesi

Abstract

Cancer is a group of diseases involving abnormal cell growth with the potential to invade or spread to other parts of the body. Investigation show that glutathione and related enzymes are often overexpressed in tumor cells and are regarded as a contributor to their drug resistance. The GST P1-1 isozyme of glutathione S-tranferases family (GST) is thought to play an important role in cancer progression and resistance to chemotherapy because it is frequently overexpressed in cancer cells and drug-resistant tumors. The available literature contains a limited number of strong GST P1-1 inhibitors. Additionally, in vivo studies of these inhibitors are highly restricted because of their toxic effects. Therefore, the most potent and comparatively less harmful GST P1-1 inhibitors seem to be an attractive target for drug development. The aim of this study is to evaluate the protective effects of chlorophylline as an antioxidant molecule which has inhibitory effects on GST P1-1 on chemically-induced breast cancer model. For this purpose, N-methyl nitrosourea (MNU) was used for inducing carcinogenesis in female Sprague-Dawley rats. Their weight and tumor diameters were measured throughout 5 months. At the end of the study, all animals were sacrificed and GST activities in liver and tumor tissues were determined. Glutathione levels and DNA damage were also determined in blood, liver and tumor tissues. As a result, it was observed that chlorophylline treatment increased glutahione level in liver because of its antioxidant effect. and significantly decreased the DNA damage in blood, liver and tumor tissues (P<0.05).